Skip Navigation


JPR Advance Access originally published online on March 8, 2008
Journal of Plankton Research 2008 30(7):755-763; doi:10.1093/plankt/fbn035
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
30/7/755    most recent
fbn035v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Band-Schmidt, C. J.
Right arrow Articles by Hernández-Saavedra, N. Y.
PubMed
Right arrow Articles by Band-Schmidt, C. J.
Right arrow Articles by Hernández-Saavedra, N. Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Heterogeneity of LSU rDNA sequences and morphology of Gymnodinium catenatum dinoflagellate strains in Bahía Concepción, Gulf of California, Mexico

Christine J. Band-Schmidt1,*, Delia I. Rojas-Posadas2, Lourdes Morquecho2 and Norma Y. Hernández-Saavedra2

1 Departamento de Plancton y Ecología Marina, Centro Interdisciplinario de Ciencias Marinas-IPN, AP 592, La Paz, BCS 23000, Mexico 2 Centro de Investigaciones BiolÓgicas del Noroeste, AP 128, La Paz, BCS 23000, Mexico

* CORRESPONDING AUTHOR: cbands{at}ipn.mx or cjband{at}yahoo.com

Received on November 12, 2007; accepted on March 4, 2008


  Abstract

We report the large subunit (LSU) rDNA sequences and morphology of several Gymnodinium catenatum dinoflagellate strains isolated from Bahía Concepción, Mexico. PCR amplifications of the D1–D2 fragment of the nuclear LSU rDNA gene resulted in a single product of ~889 bp. A phylogenetic tree was constructed from the partial LSU rDNA (350 bp) sequences from six strains of G. catenatum isolated from the bay and 12 isolates from around the world. Strains from the bay grouped within the G. catenatum clade; however, a constant characteristic of G. catenatum strains from the bay is that, at position {approx}453, a single nucleotide polymorphism was observed, presenting cytosine instead of guanine. This single base polymorphism could indicate a mutation or genetic isolation from other G. catenum populations. It is suggested that the Western Pacific could be the ancestral population of this species. Significant differences were found between cell length (CL), cell width (CW) and CW/CL ratios in laboratory cultures in the late exponential growth phase. Average CL varied between 41 and 53.69 µm, with a range between 26.94 and 69.99 µm; average CW varied between 33.75 and 40.68 µm, with a range between 25.04 and 54.73 µm; the average CW/CL varied between 0.76 and 0.97 µm, with a range between 0.49 and 1.27 µm. Cingulum thickness was between 3 and 4.11 µm, with a range between 1.97 and 6.74 µm. Cell size varied with the length of the chain and position in the chain.

Corresponding editor: Roger Harris


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.